p16(INK4A) expression in invasive laryngeal cancer

Brenda Y Hernandez; Mobeen Rahman; Charles F Lynch; Wendy Cozen; Elizabeth R Unger; Martin Steinau; Trevor Thompson; Maria Sibug Saber; Sean F Altekruse; Marc T Goodman; Amy Powers; Christopher Lyu; Mona Saraiya

doi:10.1016/j.pvr.2016.03.001

p16(INK4A) expression in invasive laryngeal cancer

Papillomavirus Res. 2016 Dec:2:52-55. doi: 10.1016/j.pvr.2016.03.001. Epub 2016 Mar 9.

Authors

Affiliations

¹ University of Hawaii Cancer Center, 701 Ilalo Street #239, Honolulu, Hawaii, USA 96813.
² University of Hawaii John A. Burns School of Medicine, 651 Ilalo Street #411E, Honolulu, Hawaii, USA 96813.
³ The University of Iowa, College of Public Health, Department of Epidemiology, 145 North Riverside Drive S447 CPHB, Iowa City, Iowa, USA 52242-2007.
⁴ University of Southern California, Norris Comprehensive Cancer Center and Keck School of Medicine, Departments of Preventive Medicine and Pathology, 1441 Eastlake Avenue MC 9175, Los Angeles, California, USA 90089-9175.
⁵ Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases, Division of High-Consequence Pathogens and Pathology, Atlanta, Georgia, USA 30329-4027.
⁶ Centers for Disease Control and Prevention, 4770 Buford Highway NE, Mailstop F-76, Atlanta, Georgia, USA 30341-3717.
⁷ University of Southern California, Norris Comprehensive Cancer Center, 1441 Eastlake Avenue #4420, Los Angeles, California, USA 90089-9175.
⁸ National Cancer Institute, Surveillance Research Program, 9609 Medical Center Drive, Room 4E536, Rockville, Maryland, USA 20850.
⁹ Cedars-Sinai Medical Center, 8700 Beverly Blvd, Room 1S37, Los Angeles, California, USA 90048.
¹⁰ The Queen's Medical Center, Department of Pathology, 1301 Punchbowl St., Iolani 4, Honolulu, Hawaii, USA 96813.
¹¹ Battelle, 100 Capitola Drive, Suite 200, Durham, North Carolina, USA 27713.
¹² Centers for Disease Control and Prevention, 4770 Buford Highway NE, Atlanta, Georgia, USA 30341-3717.

Abstract

We examined p16 expression in tumors from a population-based sample of laryngeal cancer cases diagnosed in the U.S. Samples had been previously genotyped for HPV DNA. Overall, p16 expression was observed in laryngeal tissue from 8 of 101 (7.9%) cases. p16 expression was observed in 2 of 16 (12.5%) cases previously determined to be HPV DNA positive. The two cases dually positive for p16 and HPV DNA were non-keratinizing SCC and papillary SCC tumors that were positive for genotypes 18 and 35/89, respectively. Positivity for p16 and/or HPV DNA was not associated with 5-year survival (log-rank p value= 0.55). Our findings support a limited role of HPV in laryngeal carcinogenesis. p16 is not a reliable surrogate for HPV status in laryngeal cancers and is not a predictor of laryngeal cancer survival.

Keywords: HPV; human papillomavirus; laryngeal cancer; larynx; p16; p16(INK4A); survival.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Aged
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / pathology
Carcinoma, Squamous Cell / virology
Cyclin-Dependent Kinase Inhibitor p16 / genetics*
Cyclin-Dependent Kinase Inhibitor p16 / metabolism
DNA, Viral / genetics
Female
Gene Expression*
Genotype
Human papillomavirus 16 / genetics
Human papillomavirus 16 / isolation & purification
Humans
Laryngeal Neoplasms / diagnosis
Laryngeal Neoplasms / genetics*
Laryngeal Neoplasms / pathology*
Laryngeal Neoplasms / virology*
Male
Middle Aged
Neoplasm Invasiveness
Papillomavirus Infections / complications
Papillomavirus Infections / virology
Survival Analysis
United States / epidemiology

Substances

Cyclin-Dependent Kinase Inhibitor p16
DNA, Viral
p12(INK4a) protein, human

Abstract

Publication types

MeSH terms

Substances

Grants and funding