ZIF-67 derived porous Co3O4 hollow nanopolyhedron functionalized solution-gated graphene transistors for simultaneous detection of glucose and uric acid in tears

Can Xiong; Tengfei Zhang; Weiyu Kong; Zhixiang Zhang; Hao Qu; Wei Chen; Yanbo Wang; Linbao Luo; Lei Zheng

doi:10.1016/j.bios.2017.10.004

ZIF-67 derived porous Co₃O₄ hollow nanopolyhedron functionalized solution-gated graphene transistors for simultaneous detection of glucose and uric acid in tears

Biosens Bioelectron. 2018 Mar 15:101:21-28. doi: 10.1016/j.bios.2017.10.004. Epub 2017 Oct 5.

Authors

Can Xiong¹, Tengfei Zhang², Weiyu Kong², Zhixiang Zhang², Hao Qu³, Wei Chen¹, Yanbo Wang⁴, Linbao Luo², Lei Zheng⁵

Affiliations

¹ School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China.
² School of Electronic Science and Applied Physics, Hefei University of Technology, Hefei 230009, China.
³ School of Biological and Medical Engineering, Hefei University of Technology, Hefei 230009, China; CAS Key Laboratory of Bio-inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: quhao@hfut.edu.cn.
⁴ Key Laboratory for Food Microbial Technology of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, 18 Xue Zheng Street, Hangzhou 310018, China.
⁵ School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, China. Electronic address: lzheng@hfut.edu.cn.

PMID: 29031886
DOI: 10.1016/j.bios.2017.10.004

Abstract

Biomarkers in tears have attracted much attention in daily healthcare sensing and monitoring. Here, highly sensitive sensors for simultaneous detection of glucose and uric acid are successfully constructed based on solution-gated graphene transistors (SGGTs) with two separate Au gate electrodes, modified with GOx-CHIT and BSA-CHIT respectively. The sensitivity of the SGGT is dramatically improved by co-modifying the Au gate with ZIF-67 derived porous Co₃O₄ hollow nanopolyhedrons. The sensing mechanism for glucose sensor is attributed to the reaction of H₂O₂ generated by the oxidation of glucose near the gate, while the sensing mechanism for uric acid is due to the direct electro-oxidation of uric acid molecules on the gate. The optimized glucose and uric acid sensors show the detection limits both down to 100nM, far beyond the sensitivity required for non-invasive detection of glucose and uric acid in tears. The glucose and uric acid in real tear samples was quantitatively detected at 323.2 ± 16.1μM and 98.5 ± 16.3μM by using the functionalized SGGT device. Due to the low-cost, high-biocompatibility and easy-fabrication features of the ZIF-67 derived porous Co₃O₄ hollow nanopolyhedron, they provide excellent electrocatalytic nanomaterials for enhancing sensitivity of SGGTs for a broad range of disease-related biomarkers.

Keywords: Glucose; Graphene transistors; High sensitivity; Porous Co(3)O(4) hollow nanopolyhedrons; Uric acid.

Publication types

Evaluation Study

MeSH terms

Biosensing Techniques / economics
Biosensing Techniques / instrumentation*
Cobalt / chemistry*
Equipment Design
Glucose / analysis*
Graphite / chemistry*
Humans
Limit of Detection
Nanostructures / chemistry
Nanostructures / ultrastructure
Oxides / chemistry*
Porosity
Tears / chemistry*
Transistors, Electronic* / economics
Uric Acid / analysis*

Substances

Oxides
Uric Acid
Cobalt
Graphite
Glucose
cobalt oxide