Optofluidic time-stretch quantitative phase microscopy

Baoshan Guo; Cheng Lei; Yi Wu; Hirofumi Kobayashi; Takuro Ito; Yaxiaer Yalikun; Sangwook Lee; Akihiro Isozaki; Ming Li; Yiyue Jiang; Atsushi Yasumoto; Dino Di Carlo; Yo Tanaka; Yutaka Yatomi; Yasuyuki Ozeki; Keisuke Goda

doi:10.1016/j.ymeth.2017.10.004

Optofluidic time-stretch quantitative phase microscopy

Methods. 2018 Mar 1:136:116-125. doi: 10.1016/j.ymeth.2017.10.004. Epub 2017 Oct 12.

Authors

Baoshan Guo¹, Cheng Lei², Yi Wu³, Hirofumi Kobayashi¹, Takuro Ito⁴, Yaxiaer Yalikun⁵, Sangwook Lee¹, Akihiro Isozaki¹, Ming Li⁶, Yiyue Jiang¹, Atsushi Yasumoto⁷, Dino Di Carlo⁶, Yo Tanaka⁵, Yutaka Yatomi⁷, Yasuyuki Ozeki⁸, Keisuke Goda⁹

Affiliations

¹ Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan.
² Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan. Electronic address: leicheng@chem.s.u-tokyo.ac.jp.
³ Department of Materials Science and Engineering, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
⁴ Japan Science and Technology Agency, Kawaguchi 332-0012, Japan.
⁵ Laboratory for Integrated Biodevices, Quantitative Biology Center, RIKEN, Osaka 565-0871, Japan.
⁶ Department of Bioengineering, Mechanical Engineering, and California NanoSystems Institute, University of California, Los Angeles, CA 90095, USA.
⁷ Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 113-8655, Japan.
⁸ Department of Electrical Engineering and Information Systems, University of Tokyo, Tokyo 113-8656, Japan.
⁹ Department of Chemistry, University of Tokyo, Tokyo 113-0033, Japan; Japan Science and Technology Agency, Kawaguchi 332-0012, Japan; Department of Electrical Engineering, University of California, Los Angeles, CA 90095, USA. Electronic address: goda@chem.s.u-tokyo.ac.jp.

PMID: 29031836
DOI: 10.1016/j.ymeth.2017.10.004

Abstract

Innovations in optical microscopy have opened new windows onto scientific research, industrial quality control, and medical practice over the last few decades. One of such innovations is optofluidic time-stretch quantitative phase microscopy - an emerging method for high-throughput quantitative phase imaging that builds on the interference between temporally stretched signal and reference pulses by using dispersive properties of light in both spatial and temporal domains in an interferometric configuration on a microfluidic platform. It achieves the continuous acquisition of both intensity and phase images with a high throughput of more than 10,000 particles or cells per second by overcoming speed limitations that exist in conventional quantitative phase imaging methods. Applications enabled by such capabilities are versatile and include characterization of cancer cells and microalgal cultures. In this paper, we review the principles and applications of optofluidic time-stretch quantitative phase microscopy and discuss its future perspective.

Keywords: High-throughput screening; Machine learning; Microfluidics; Optofluidic time-stretch microscopy; Quantitative phase imaging; Single-cell analysis.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Humans
Microfluidic Analytical Techniques / methods*
Microscopy / methods*
Microscopy, Phase-Contrast