Production of low molecular collagen peptides-loaded liposomes using different charged lipids

Abstract

To encapsulate water soluble collagen peptides, liposomes loaded with peptides were assembled using a combination of thin film hydration and ultrasonication emulsification techniques. The influence of lipid charge, duration and power of ultrasonication, and collagen peptide concentration were evaluated. Layered liposomes loaded with collagen peptides, charged lipids, chitosan (+) or low-methoxyl pectin (-) were produced using the layer-by-layer electrostatic deposition method. For the liposomes loaded with collagen peptides, the most efficient and dependable manufacturing method was variation of the ultrasonication duration, which was capable of producing smaller sizes (through increasing ultrasonication duration) and liposomes loaded with peptides with >60% encapsulation efficiency. For layered liposomes loaded with collagen peptides, charged lipids were determined to be more effective in the production of smaller liposomes than charged biopolymers. In addition, layered and non-layered liposomes loaded with peptides with a particle size <100nm were physically stable during storage, regardless of storage temperature and time.

Keywords: Bioactive peptide; Charged liposome; Droplet size; Layered liposome; Physical stability.