Expression of K channels in Xenopus laevis oocytes injected with poly(A+) mRNA from the insulin-secreting beta-cell line, HIT T15

FEBS Lett. 1988 Nov 7;239(2):185-9. doi: 10.1016/0014-5793(88)80913-x.

Abstract

Two types of exogenous K channel were identified in Xenopus laevis oocytes injected with poly(A+) mRNA from the insulin-secreting cell line HIT T15. One of these was the ATP-regulated K channel (G channel) as evidenced by its conductance and inhibition by tolbutamide. The other resembled the Ca-activated K channel from beta-cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / pharmacology
  • Cell Line
  • Cell Membrane / drug effects
  • Cell Membrane / physiology
  • Female
  • Insulin / metabolism
  • Insulin Secretion
  • Islets of Langerhans / metabolism
  • Membrane Potentials / drug effects
  • Oocytes / physiology*
  • Poly A / genetics*
  • Potassium Channels / physiology*
  • Protein Biosynthesis
  • RNA, Messenger / genetics*
  • Tolbutamide / pharmacology
  • Xenopus laevis

Substances

  • Insulin
  • Potassium Channels
  • RNA, Messenger
  • Poly A
  • Tolbutamide
  • Calcium