Ascosteroside D, a new mitochondrial respiration inhibitor discovered by pesticidal screening using insect ADP/ATP carrier protein-expressing Saccharomyces cerevisiae

Yoshihiro Watanabe; Yukihiro Asami; Satomi Narusawa; Shohei Hashimoto; Masato Iwatsuki; Kenichi Nonaka; Yasuo Shinohara; Takahiro Shiotsuki; Naoya Ichimaru; Hideto Miyoshi; Satoshi Ōmura; Kazuro Shiomi

doi:10.1038/ja.2017.118

Ascosteroside D, a new mitochondrial respiration inhibitor discovered by pesticidal screening using insect ADP/ATP carrier protein-expressing Saccharomyces cerevisiae

J Antibiot (Tokyo). 2017 Oct 11. doi: 10.1038/ja.2017.118. Online ahead of print.

Authors

Yoshihiro Watanabe¹, Yukihiro Asami^{1

2}, Satomi Narusawa³, Shohei Hashimoto³, Masato Iwatsuki^{1

2}, Kenichi Nonaka^{1

2}, Yasuo Shinohara⁴, Takahiro Shiotsuki⁵, Naoya Ichimaru⁶, Hideto Miyoshi⁷, Satoshi Ōmura², Kazuro Shiomi^{1

2}

Affiliations

¹ Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan.
² Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan.
³ Department of Chemistry, School of Science, Kitasato University, Kanagawa, Japan.
⁴ Institute for Genome Research, Tokushima University, Tokushima, Japan.
⁵ Insect Growth Regulation Research Unit, National Institute of Agrobiological Sciences, Ibaraki, Japan.
⁶ Faculty of Bioenvironmental Science, Kyoto Gakuen University, Kyoto, Japan.
⁷ Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.

PMID: 29018265
DOI: 10.1038/ja.2017.118

Abstract

A new lanostane-type triterpenoid, ascosteroside D, was isolated from a fungus, Aspergillus sp. FKI-6682. It inhibited insect ADP/ATP carrier protein (AAC)-expressing Saccharomyces cerevisiae in glycerol-containing medium, but did not inhibit Δaac S. cerevisiae in glucose-containing medium. It is hypothesized that ascosteroside D inhibits ATP production in mitochondria.The Journal of Antibiotics advance online publication, 11 October 2017; doi:10.1038/ja.2017.118.