Variations in yield and gluten proteins in durum wheat varieties under late-season foliar versus soil application of nitrogen fertilizer in a northern Mediterranean environment

J Sci Food Agric. 2018 Apr;98(6):2360-2369. doi: 10.1002/jsfa.8727. Epub 2017 Nov 11.

Abstract

Background: With the increasing demand for high-quality foodstuffs and concern for environmental sustainability, late-season nitrogen (N) foliar fertilization of common wheat is now an important and widespread practice. This study investigated the effects of late-season foliar versus soil N fertilization on yield and protein content of four varieties of durum wheat, Aureo, Ariosto, Biensur and Liberdur, in a three-year field trial in northern Italy.

Results: Variations in low-molecular-weight glutenins (LMW-GS), high-molecular-weight glutenins (HMW-GS) and gliadins were assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It was found that N applied to the canopy did not improve protein rate compared with N application to the soil (general mean 138 mg g-1 ), but moderately increased productivity in the high-yielding varieties Liberdur and Biensur (three-year means 7.23 vs 7.13 and 7.53 vs 7.09 t ha-1 respectively). Technological quality was mainly related to variety choice, Aureo and Ariosto having higher protein rates and glutenin/gliadin ratios. Also found was a strong 'variety × N application method' interaction in the proportions of protein subunits within each class, particularly LMW-GS and gliadins. A promising result was the higher N uptake efficiency, although as apparent balance, combined with higher HMW/LMW-GS ratio in var. Biensur.

Conclusion: Late-season foliar N fertilization allows N fertilizer saving, potentially providing environmental benefits in the rainy climate of the northern Mediterranean area, and also leads to variety-dependent up-regulation of essential LMW-GS and gliadins. Variety choice is a key factor in obtaining high technological quality, although it is currently associated with modest grain yield. This study provides evidence of high quality in the specific high-yielding variety Biensur, suggesting its potential as a mono-varietal semolina for pasta production. © 2017 Society of Chemical Industry.

Keywords: HMW-GS; LMW-GS; durum wheat varieties; foliar nitrogen fertilization; gliadins; sustainable agriculture; technological quality in pasta production.

MeSH terms

  • Climate
  • Fertilizers / analysis*
  • Glutens / analysis
  • Glutens / metabolism*
  • Italy
  • Nitrogen / analysis
  • Nitrogen / metabolism*
  • Seasons
  • Seeds / chemistry
  • Seeds / growth & development
  • Seeds / metabolism
  • Soil / chemistry
  • Triticum / chemistry
  • Triticum / growth & development
  • Triticum / metabolism*

Substances

  • Fertilizers
  • Soil
  • Glutens
  • Nitrogen