Monoamine oxidase and semicarbazide sensitive amine oxidase activities in toad liver mitochondria

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;89(2):257-9. doi: 10.1016/0742-8413(88)90220-4.

Abstract

1. The deamination of 5-HT and PEA has been assayed by a radiochemical method in mitochondria isolated from toad liver. 2. Time courses of 5-HT and PEA deamination indicate that when PEA is used as the substrate, higher specific activities are obtained. 3. 5-HT is deaminated by MAO A and partially by a SSAO-like enzyme. 4. PEA is deaminated exclusively by SSAO and, MAO B activity, at least under the adopted experimental conditions, is not detectable.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bufo bufo
  • In Vitro Techniques
  • Mitochondria, Liver / drug effects
  • Mitochondria, Liver / enzymology*
  • Monoamine Oxidase / metabolism*
  • Monoamine Oxidase Inhibitors / pharmacology
  • Phenethylamines / metabolism
  • Semicarbazides / pharmacology*
  • Serotonin / metabolism

Substances

  • Monoamine Oxidase Inhibitors
  • Phenethylamines
  • Semicarbazides
  • Serotonin
  • Monoamine Oxidase