MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation

Alan Van Goethem; Pieter Mestdagh; Tom Van Maerken; Jo Vandesompele

doi:10.1007/978-1-4939-7231-9_13

MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation

Methods Mol Biol. 2017:1654:197-208. doi: 10.1007/978-1-4939-7231-9_13.

Authors

Alan Van Goethem¹, Pieter Mestdagh¹, Tom Van Maerken¹, Jo Vandesompele²

Affiliations

¹ Center for Medical Genetics, Ghent University, Ghent, Belgium.
² Center for Medical Genetics, Ghent University, Ghent, Belgium. joke.vandesompele@ugent.be.

PMID: 28986791
DOI: 10.1007/978-1-4939-7231-9_13

Abstract

miRNAs are small noncoding RNA molecules that function as regulators of gene expression. Deregulated miRNA expression has been reported in various diseases including cancer. Due to their small size and high degree of homology, accurate quantification of miRNA expression is technically challenging. In this chapter, we present two different technologies for miRNA quantification: small RNA sequencing and RT-qPCR.

Keywords: Normalization; RT-qPCR; Small RNA sequencing; miRNA; miRNA annotation.

MeSH terms

Gene Expression Profiling / methods
Humans
MicroRNAs / genetics*
RNA, Small Untranslated / genetics
Real-Time Polymerase Chain Reaction / methods*
Sequence Analysis, RNA / methods*

Substances

MicroRNAs
RNA, Small Untranslated