GPR120 is not required for ω-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells

Shenglong Zhu; Xiaowei Jiang; Siyuan Jiang; Guangxiao Lin; Jianping Gong; Wei Chen; Zhao He; Yong Q Chen

doi:10.1002/cbin.10883

GPR120 is not required for ω-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells

Cell Biol Int. 2018 Feb;42(2):180-186. doi: 10.1002/cbin.10883. Epub 2017 Dec 15.

Authors

Shenglong Zhu^{1

2

3}, Xiaowei Jiang⁴, Siyuan Jiang⁵, Guangxiao Lin^{1

2}, Jianping Gong⁴, Wei Chen^{1

2

6

7}, Zhao He^{1

2

3}, Yong Q Chen^{1

2

3

6

8}

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.
² School of Food Science and Technology, Jiangnan University, Wuxi, P. R. China.
³ Wuxi Medical School, Jiangnan University, Wuxi, P. R. China.
⁴ Chongqing Key Laboratory of Hepatobiliary Surgery and Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, P. R. China.
⁵ CAS Key Laboratory of Bio-medical Diagnostic, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, P. R. China.
⁶ National Engineer Research Center for Functional Food, Jiangnan University, Wuxi, P. R. China.
⁷ Beijing Innovation Center of Food Nutrition and Human Health, Beijing Technology and Business University, Beijing, P. R. China.
⁸ School of Medicine, Wake Forest University, North Carolina, USA.

PMID: 28980737
DOI: 10.1002/cbin.10883

Abstract

Intake of ω-3 PUFAs reduces the frequency of breast cancer, and GPR120 receptor transduces ω-3 PUFAs signaling to increase insulin sensitivity in mice, but whether GPR120 mediates ω-3 PUFAs signaling to inhibit breast carcinogenesis is currently unknown. In the present study, we found that GPR120 is highly expressed in human breast cancerous tissues but not adjacent normal tissue. Knockdown of GPR120 by siRNA in breast cancer cells significantly reduced cell growth, and dramatically increased ω-3 FFA-induced cell growth inhibition and apoptosis. Thus, these observations indicated that GPR120 promotes breast cancer cell growth, whereas ω-3 PUFA-induce breast cancer cell apoptosis independently of GPR120.

Keywords: GPR120; breast cancer; ω-3 PUFAs.

MeSH terms

Antineoplastic Agents / therapeutic use*
Apoptosis
Breast Neoplasms / drug therapy*
Breast Neoplasms / metabolism
Breast Neoplasms / pathology
Cell Line, Tumor
Cell Proliferation / drug effects
Fatty Acids, Omega-3 / therapeutic use*
Female
Humans
Receptors, G-Protein-Coupled / metabolism*
Receptors, G-Protein-Coupled / physiology

Substances

Antineoplastic Agents
FFAR4 protein, human
Fatty Acids, Omega-3
Receptors, G-Protein-Coupled