Avidity as a criterion for diagnosis of human strongyloidiasis increases specificity of IgG ELISA

Diagn Microbiol Infect Dis. 2017 Dec;89(4):262-264. doi: 10.1016/j.diagmicrobio.2017.08.008. Epub 2017 Aug 26.

Abstract

This study evaluates the inclusion of the IgG avidity index in ELISA to detect anti-Strongyloides stercoralis IgG. The ELISA index revealed 70% of specificity. With the inclusion of screening AI, specificity increased to 80%. IgG avidity complemented traditional IgG ELISA by eliminating some of the suspected or false positive cases.

Keywords: Avidity-ELISA; Cross-reaction; Strongyloides sp.; Strongyloidiasis.

MeSH terms

  • Animals
  • Antibodies, Helminth / blood
  • Antibody Affinity*
  • Antigens, Helminth / blood
  • Enzyme-Linked Immunosorbent Assay*
  • Humans
  • Immunoglobulin G / blood*
  • Sensitivity and Specificity
  • Strongyloides / immunology
  • Strongyloides / isolation & purification
  • Strongyloidiasis / diagnosis*
  • Strongyloidiasis / immunology

Substances

  • Antibodies, Helminth
  • Antigens, Helminth
  • Immunoglobulin G