Abstract
A versatile bio-logic system is proposed using a surface charge tunable fluorescent protein (H39GFP) as a generic processor. On the basis of the cell penetration of H39GFP triggered by H+ and metal ions, OR logic gates are successfully operated in living cells with transfection of functional nucleic acids (Cy5 labelled DNA and siRNA) as the readout, as well as RNA interference.
MeSH terms
-
Carbocyanines / chemistry
-
DNA / chemistry*
-
DNA / metabolism
-
Dynamic Light Scattering
-
Green Fluorescent Proteins / chemistry*
-
HeLa Cells
-
Humans
-
Hydrogen-Ion Concentration
-
Metals / chemistry
-
Microscopy, Confocal
-
RNA, Small Interfering / chemistry*
-
RNA, Small Interfering / metabolism
-
Transfection / methods
Substances
-
Carbocyanines
-
Metals
-
RNA, Small Interfering
-
cyanine dye 5
-
Green Fluorescent Proteins
-
DNA