Quantification of collagen organization in histopathology samples using liquid crystal based polarization microscopy

Adib Keikhosravi; Yuming Liu; Cole Drifka; Kaitlin M Woo; Amitabh Verma; Rudolf Oldenbourg; Kevin W Eliceiri

doi:10.1364/BOE.8.004243

Quantification of collagen organization in histopathology samples using liquid crystal based polarization microscopy

Biomed Opt Express. 2017 Aug 29;8(9):4243-4256. doi: 10.1364/BOE.8.004243. eCollection 2017 Sep 1.

Authors

Adib Keikhosravi^{1

2

3

4}, Yuming Liu^{1

4}, Cole Drifka^{1

2

3}, Kaitlin M Woo⁵, Amitabh Verma⁶, Rudolf Oldenbourg^{6

7}, Kevin W Eliceiri^{1

2

3}

Affiliations

¹ Laboratory for Optical and Computational Instrumentation, University of Wisconsin at Madison, Madison WI, USA.
² Biomedical Engineering Department, University of Wisconsin at Madison, Madison WI, USA.
³ Morgridge Institute for Research, Madison WI, USA.
⁴ These authors have contributed equally.
⁵ Department of Biostatistics and Medical informatics, Brown University, Providence, RI, USA.
⁶ Marine Biological Laboratory, Woods Hole, MA, USA.
⁷ Department of Physics, Brown University, Providence, RI, USA.

Abstract

A number of histopathology studies have utilized the label free microscopy method of Second Harmonic Generation (SHG) to investigate collagen organization in disease onset and progression. Here we explored an alternative label free imaging approach, LC-PolScope that is based on liquid crystal based polarized light imaging. We demonstrated that this more accessible technology has the ability to visualize all fibers of interest and has a good to excellent correlation between SHG and LC-PolScope measurements in fibrillar collagen orientation and alignment. This study supports that LC-PolScope is a viable alternative to SHG for label free collagen organization measurements in thin histology sections.

Keywords: (100.2960) Image analysis; (110.0180) Microscopy; (110.1758) Computational imaging; (190.2620) Harmonic generation and mixing; (260.5430) Polarization.

Abstract

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