The memory lymphocyte immunostimulation assay in immune system disorders: Is useful or useless?

Maria Vadalà; Carmen Laurino; Beniamino Palmieri

doi:10.4103/JLP.JLP_137_16

The memory lymphocyte immunostimulation assay in immune system disorders: Is useful or useless?

J Lab Physicians. 2017 Oct-Dec;9(4):223-226. doi: 10.4103/JLP.JLP_137_16.

Authors

Maria Vadalà^{1

2}, Carmen Laurino^{1

2}, Beniamino Palmieri²

Affiliations

¹ Department of General Surgery and Surgical Specialties, University of Modena and Reggio Emilia Medical School, Surgical Clinic, Modena, Italy.
² Network of the Second Opinion, Modena, Italy.

Abstract

Aim: The aim of the study was to evaluate the clinical relevance, sensitivity and specificity of in vitro blood test, Memory Lymphocyte ImmunoStimulation Assay (MELISA^®), in genetically predisposed patients that suffer by autoimmune/inflammatory syndrome induced by adjuvants, after HPV-vaccination and that could have a high metal hypersensitivity.

Materials and methods: Sixteen girls (aged 12-24 years) that developed long-lasting and invalidating somatoform symptoms occurring within 20 days postvaccination are included in this descriptive study. The hypersensitivity to five metals (aluminum, nickel, mercury, methyl mercury, and thimerosal) was measured by MELISA^® test.

Results: Seven girls showed negativity to all the five metals tested. The findings showed metal hypersensitivity only in nine patients: Toxicity to aluminum (two girls), reactivity to nickel (seven girls), followed by mercury (seven girls).

Conclusion: The MELISA^® assay is neither sensitive nor specific in detecting metal hypersensitivity and associated chronic diseases, including autoimmune pathologies.

Keywords: Hypersensitivity; Memory Lymphocyte ImmunoStimulation Assay; metal; test.