Visualizing Nuclear RNA Editing

Hodaya Hochberg; Yaron Shav-Tal

doi:10.1016/j.tibs.2017.09.004

Visualizing Nuclear RNA Editing

Trends Biochem Sci. 2017 Nov;42(11):845-847. doi: 10.1016/j.tibs.2017.09.004. Epub 2017 Sep 28.

Authors

Hodaya Hochberg¹, Yaron Shav-Tal²

Affiliations

¹ The Mina & Everard Goodman Faculty of Life Sciences & Institute of Nanotechnology, Bar-Ilan University, Ramat Gan 5290002, Israel.
² The Mina & Everard Goodman Faculty of Life Sciences & Institute of Nanotechnology, Bar-Ilan University, Ramat Gan 5290002, Israel. Electronic address: Yaron.Shav-Tal@biu.ac.il.

PMID: 28965669
DOI: 10.1016/j.tibs.2017.09.004

Abstract

RNA editing results in the site-specific conversion of adenosine to inosine in mRNAs. Genomics has revealed millions of editing sites in metazoans, but examining the spatial aspects of editing in cells has been challenging. A new method, inosineFISH (inoFISH), provides the ability to detect edited and unedited mRNAs within intact cells.

Keywords: RNA FISH; RNA editing; nuclear retention; paraspeckles.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Comment

MeSH terms

Animals
Cell Nucleus / genetics*
Humans
In Situ Hybridization, Fluorescence
Inosine / chemistry
RNA Editing / genetics*
RNA, Messenger / genetics*

Substances

RNA, Messenger
Inosine