Serum quantitative proteomic analysis of patients with keshan disease based on iTRAQ labeling technique: A first term study

Abstract

Keshan disease (KD), an endemic myocardiopathy, with unknown etiology, is still threatening human health. Proteomics studies of Keshan disease is rarely known. In the current study, isobaric tags for relative and absolute quantitation (iTRAQ) labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was firstly used to screen for the differentially expressed proteins (DEPs) in serum between Keshan disease and healthy residents in endemic area. 27 differentially expressed proteins were quantified, 9 of which were significantly altered (fold change > 1.2 or < 0.8) between the two groups. Bioinformatic analysis revealed that a wide variety of biology process played roles in KD pathophysiology. These differentially expressed proteins were mainly involved in complement coagulation pathways. A significant high serum level of LGALS3BP were observed. Our first term study in KD proteomics provided new view into the molecular mechanisms of KD disease.

Keywords: Biomarker; DEPs; Keshan disease; Proteomic analysis; iTRAQ.