Tools for the cryo-EM gold rush: going from the cryo-EM map to the atomistic model

Doo Nam Kim; Karissa Y Sanbonmatsu

doi:10.1042/BSR20170072

Tools for the cryo-EM gold rush: going from the cryo-EM map to the atomistic model

Biosci Rep. 2017 Dec 5;37(6):BSR20170072. doi: 10.1042/BSR20170072. Print 2017 Dec 22.

Authors

Doo Nam Kim¹, Karissa Y Sanbonmatsu^{2

3}

Affiliations

¹ Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, U.S.A.
² Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, U.S.A. kys@lanl.gov.
³ New Mexico Consortium, Los Alamos, U.S.A.

Abstract

As cryo-electron microscopy (cryo-EM) enters mainstream structural biology, the demand for fitting methods is high. Here, we review existing flexible fitting methods for cryo-EM. We discuss their importance, potential concerns and assessment strategies. We aim to give readers concrete descriptions of cryo-EM flexible fitting methods with corresponding examples.

Keywords: cryo electron microscopy; cryo-EM; modeling; structural biology.

Publication types

Review

MeSH terms

Cryoelectron Microscopy / methods*
Humans
Models, Molecular
Protein Conformation
Proteins / chemistry*
Proteins / ultrastructure

Substances

Proteins

Grants and funding

R01 GM072686/GM/NIGMS NIH HHS/United States