Specific titin and myomesin domains stimulate myoblast proliferation

Irina V Kravchenko; Vladimir A Furalyov; Vladimir O Popov

doi:10.1016/j.bbrep.2016.12.007

Specific titin and myomesin domains stimulate myoblast proliferation

Biochem Biophys Rep. 2016 Dec 30:9:226-231. doi: 10.1016/j.bbrep.2016.12.007. eCollection 2017 Mar.

Authors

Irina V Kravchenko¹, Vladimir A Furalyov¹, Vladimir O Popov¹

Affiliation

¹ Federal Research Centre of Biotechnology, Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskiy prospect 33, 119071 Moscow, Russia.

Abstract

Myofibrillar proteins titin and myomesin stimulated myoblast proliferation as determined by MTT-test and labelled thymidine incorporation in the DNA. Specific Fn type III and Ig-like domains of these proteins were able to exert mitogenic effects as well. Proliferative effect of Fn type III domains was highly sensitive to inhibition of Ca²⁺/calmodulin dependent protein kinase, whereas the effect of Ig-like domains showed greater sensitivity to the inhibition of adenylyl cyclase - cAMP - PKA pathway. IGF-1 autocrine signalling inhibition partially suppressed mitogenic effects revealed by both domain types.

Keywords: Fn type III domain; IGF-1; Ig-like domain; Myoblast proliferation; Myomesin; Titin.