To improve the efficiency of enzymatic saccharification for lignocellulose, an efficient crude multi-enzyme was produced by Trichoderma reesei using corncob, a low cost inducer. Expression of cbh1, bgl1, egl1, xyn1 and positive regulator xyr1 induced by corncob increased significantly compared to that by cellulose. After 120 h induction by corncob, enzymatic activities on filter, CMC, β-glucose and xylan increased 86.5, 46.9, 120.9 and 291.2% compared to those induced by cellulose, and the concentration of secreted protein increased by 120.8%. FPase:β-glucosidase and FPase:xylanase values in crude multi-enzyme I (ECI, induced by corncob) were higher than that in crude multi-enzyme II (ECII, induced by cellulose). Under the same hydrolysis conditions, the volume dosage of ECI was only half of ECII, but ECI still showed a maximum of 12.5 and 33.4% higher than ECII in the total reducing sugar and glucose yield in lignocellulose hydrolysis. Corncob could be a candidate for low cost production of multi-enzyme for efficient lignocellulose degradation, and this work could guide the genetic modification of T. reesei to obtain efficient multi-enzyme for lignocellulose hydrolysis.
Keywords: Cellulose; Corncob; Lignocellulose; Multi-enzyme; Trichoderma reesei.