A highly sensitive electrochemical biosensor based on AuNP-modified gold electrodes for selective determination of serum levels of crosslaps

Patricia Khashayar; Ghassem Amoabediny; Bagher Larijani; Morteza Hosseini; Rik Verplancke; Michel De Keersmaecker; Annemie Adriaens; Stefan Goemaere; Tom Fiers; Jan Vanfleteren

doi:10.1007/s13205-017-0919-y

A highly sensitive electrochemical biosensor based on AuNP-modified gold electrodes for selective determination of serum levels of crosslaps

3 Biotech. 2017 Oct;7(5):312. doi: 10.1007/s13205-017-0919-y. Epub 2017 Sep 13.

Authors

Patricia Khashayar^{1

2

3}, Ghassem Amoabediny^{4

5}, Bagher Larijani⁶, Morteza Hosseini¹, Rik Verplancke², Michel De Keersmaecker⁷, Annemie Adriaens⁷, Stefan Goemaere⁸, Tom Fiers⁸, Jan Vanfleteren²

Affiliations

¹ Nanobiotechnology Department, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
² Center for Microsystems Technology, IMEC and Ghent University, Zwijnaarde, Ghent, Belgium.
³ Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
⁴ Department of Biotechnology, Faculty of Chemical Engineering, School of Engineering, University of Tehran, Tehran, Iran.
⁵ Nanobiotechnology Department, Research Center for New Technology in Life Sciences Engineering, University of Tehran, Tehran, Iran.
⁶ Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute (EMRI), Shariati Hospital, Tehran University of Medical Sciences, Kargar St., Tehran, Iran.
⁷ Department of Analytical Chemistry, Ghent University, Krijgslaan 281 (S12), Ghent, Belgium.
⁸ Unit for Osteoporosis and Metabolic Bone Diseases, Ghent University Hospital, Ghent, Belgium.

Abstract

This article explains a step-wise protocol to develop an electrochemical sensor to quantify serum levels of C-telopeptide (CTX) crosslinks also known as crosslaps in a matter of minutes and with high level of accuracy. The technique needs only one-step (incubation) and can thus be used for point of care screening. Due to the excellent electrical properties of the as-prepared immunosensor, CTX levels were successfully measured from 1 to 1000 pg/mL. This is while the normal reference of the marker is 50-450 pg/mL, suggesting that the sensor can acceptably detect CTX. The results also showed a good correlation with ECLIA in measuring serum levels of CTX.

Keywords: Biosensor; Bone turnover marker; CTX; Crosslaps; Electrochemistry.