A glass fibre membrane platform that allows quantification of circulating cTnT with a LoD of 0.87 pg mL-1 is described. The proposed platform uses a glass fibre membrane, DNA-guided detection method, and antibody-conjugated fluorescent beads for the quantification of cTnT in the analytical detection range of 1-120 pg mL-1 at room temperature in 30 min. Glass fibre membranes were chemically modified to immobilize the oligonucleotide probes that catch a biomolecular complex (FB-dAB-cTnT-cAB-DNA) containing complementary oligonucleotides. There were no interferences from human cTnI, cTnC, skTnT, biotin, and hemoglobin (each 1 μg mL-1). The linearity in the serial dilution test of plasma samples indicates that this platform is highly applicable for regular health check-up to assess the risk of AMI and HF.