Abstract
Arctigenin (ARC) is a lignan that is abundant in Asteraceae plants, which show anti-inflammatory and anti-cancer activities. The current study investigated whether ARC affects cancer progression and metastasis, focusing on EMT using invasive human non-small cell lung cancer (NSCLC) cells. No toxicity was observed in the cells treated with different doses of ARC (12-100 μM). The treatment of ARC repressed TGF-β-stimulated changes of metastatic morphology and cell invasion and migration. ARC inhibited TGF-β-induced phosphorylation and transcriptional activity of smad2/3, and expression of snail. ARC also decreased expression of N-cadherin and increased expression of E-cadherin in dose-dependent and time-dependent manners. These changes were accompanied by decreased amount of phospho-smad2/3 in nucleus and nuclear translocation of smad2/3. Moreover, ARC repressed TGF-β-induced phosphorylation of ERK and transcriptional activity of β-catenin. Our data demonstrate anti-metastatic activity of ARC in lung cancer model.
Keywords:
Arctigenin; EMT; Lung cancer; Metastasis.
Copyright © 2017 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antineoplastic Agents, Phytogenic / chemistry
-
Antineoplastic Agents, Phytogenic / pharmacology*
-
Asteraceae / chemistry
-
Cadherins / metabolism
-
Carcinoma, Non-Small-Cell Lung / drug therapy*
-
Carcinoma, Non-Small-Cell Lung / metabolism
-
Carcinoma, Non-Small-Cell Lung / pathology
-
Cell Line, Tumor
-
Cell Movement / drug effects
-
Cell Survival / drug effects
-
Epithelial-Mesenchymal Transition / drug effects*
-
Furans / chemistry
-
Furans / pharmacology*
-
Humans
-
Lignans / chemistry
-
Lignans / pharmacology*
-
Lung / drug effects
-
Lung / metabolism
-
Lung / pathology
-
Lung Neoplasms / drug therapy*
-
Lung Neoplasms / metabolism
-
Lung Neoplasms / pathology
-
Neoplasm Invasiveness / pathology
-
Neoplasm Invasiveness / prevention & control
-
Phosphorylation / drug effects
-
Smad2 Protein / metabolism
-
Smad3 Protein / metabolism
-
Transforming Growth Factor beta / metabolism*
-
beta Catenin / metabolism
Substances
-
Antineoplastic Agents, Phytogenic
-
Cadherins
-
Furans
-
Lignans
-
SMAD2 protein, human
-
SMAD3 protein, human
-
Smad2 Protein
-
Smad3 Protein
-
Transforming Growth Factor beta
-
beta Catenin
-
arctigenin