Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry (Nano-PALDI MS) with Py-Tag for the Analysis of Small Molecules

Yukina Tatsuta; Yukie Tanaka; Akari Ikeda; Shigeru Matsukawa; Hajime Katano; Shu Taira

doi:10.5702/massspectrometry.S0069

Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry (Nano-PALDI MS) with Py-Tag for the Analysis of Small Molecules

Mass Spectrom (Tokyo). 2017;6(Spec Iss 2):S0069. doi: 10.5702/massspectrometry.S0069. Epub 2017 Sep 15.

Authors

Yukina Tatsuta¹, Yukie Tanaka², Akari Ikeda³, Shigeru Matsukawa², Hajime Katano¹, Shu Taira¹

Affiliations

¹ Department of Bioscience, Fukui Prefectural University.
² University of Fukui.
³ Taiyo Nippon Sanso Co.

Abstract

We compared two ionization methods, matrix assisted laser desorption/ionization (MALDI) and nanoparticle assisted laser desorption/ionization (Nano-PALDI) mass spectrometry (MS), for the analysis of amino acids derivatized with Py-Tag™ that consists pyrylium-based compound. Py-Tag is a useful stable derivatization reagent due to wide mass differences (using ¹³C as the sole stable labelling isotope). For Py-Tag labelled lysine, sensitive signals that showed less noise with a ten times higher sensitivity, showed a wider mass difference by Nano-PALDI MS compared to MALDI MS.

Keywords: LC-MS; Py-Tag; proteomics; stable isotope.