Changes in monoamine oxidase activity by mitochondria isolated from late embryos of Bufo bufo

Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;89(1):5-9. doi: 10.1016/0742-8413(88)90138-7.

Abstract

1. Monoamine oxidase activity has been assayed in mitochondria isolated from post-neural embryos (stages 14-25) of Bufo bufo, using 5-HT and PEA as substrates. 2. Mitochondria isolated from stages 19 to 25 show an increasing ability in deaminating monoamines, PEA being metabolized at a higher level with respect to 5-HT. 3. At all the examined stages 5-HT is metabolized by an enzyme corresponding to MAO A, while PEA, from stage 19, is largely deaminated by a semicarbazide-sensitive amine oxidase (SSAO). 4. The effect of Triton X-100 on MAO A activity appears remarkably different in mitochondria isolated from embryos at stages 14 and 25 respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bufo bufo / embryology
  • Bufo bufo / metabolism*
  • Embryo, Nonmammalian / metabolism
  • Mitochondria / enzymology*
  • Monoamine Oxidase / metabolism*
  • Monoamine Oxidase Inhibitors / pharmacology
  • Octoxynol
  • Phenethylamines
  • Polyethylene Glycols / pharmacology
  • Serotonin

Substances

  • Monoamine Oxidase Inhibitors
  • Phenethylamines
  • phenethylamine
  • Serotonin
  • Polyethylene Glycols
  • Octoxynol
  • Monoamine Oxidase