High sensitivity cardiac troponin I detection in physiological environment using AlGaN/GaN High Electron Mobility Transistor (HEMT) Biosensors

Indu Sarangadharan; Abiral Regmi; Yen-Wen Chen; Chen-Pin Hsu; Pei-Chi Chen; Wen-Hsin Chang; Geng-Yen Lee; Jen-Inn Chyi; Shu-Chu Shiesh; Gwo-Bin Lee; Yu-Lin Wang

doi:10.1016/j.bios.2017.09.018

High sensitivity cardiac troponin I detection in physiological environment using AlGaN/GaN High Electron Mobility Transistor (HEMT) Biosensors

Biosens Bioelectron. 2018 Feb 15:100:282-289. doi: 10.1016/j.bios.2017.09.018. Epub 2017 Sep 14.

Authors

Affiliations

¹ Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan R.O.C.
² Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan R.O.C.
³ Department of Electrical Engineering, National Central University, Jhongli City, Taoyuan County 320, Taiwan R.O.C.
⁴ Department of Medical Laboratory Science and Biotechnology, National Cheng-Kung University, Tainan City 701, Taiwan R.O.C.
⁵ Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan R.O.C; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan R.O.C; Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan R.O.C.
⁶ Institute of NanoEngineering and Microsystems, National Tsing Hua University, Hsinchu 300, Taiwan R.O.C; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 300, Taiwan R.O.C. Electronic address: ylwang@mx.nthu.edu.tw.

PMID: 28942210
DOI: 10.1016/j.bios.2017.09.018

Abstract

In this study, we report the development of a high sensitivity assay for the detection of cardiac troponin I using electrical double layer gated high field AlGaN/GaN HEMT biosensor. The unique gating mechanism overcomes the drawback of charge screening seen in traditional FET based biosensors, allowing detection of target proteins in physiological solutions without sample processing steps. Troponin I specific antibody and aptamer are used as receptors. The tests carried out using purified protein solution and clinical serum samples depict high sensitivity, specificity and wide dynamic range (0.006-148ng/mL). No additional wash or sample pre-treatment steps are required, which greatly simplifies the biosensor system. The miniaturized HEMT chip is packaged in a polymer substrate and easily integrated with a portable measurement unit, to carry out quantitative troponin I detection in serum samples with < 2µl sample volume in 5min. The integrated prototype biosensor unit demonstrates the potential of the method as a rapid, inexpensive, high sensitivity CVD biomarker assay. The highly simplified protocols and enhanced sensor performance make our biosensor an ideal choice for point of care diagnostics and personal healthcare systems.

Keywords: Electrical double layer gated AlGaN/GaN HEMT biosensor; High sensitivity cardiac Troponin I detection; Personal healthcare; Point of care diagnostics.

Publication types

Evaluation Study

MeSH terms

Aluminum Compounds / chemistry*
Antibodies, Immobilized / chemistry
Biomarkers / analysis
Biomarkers / blood
Biosensing Techniques / instrumentation*
Biosensing Techniques / methods
Electrons
Equipment Design
Gallium / chemistry*
Humans
Point-of-Care Systems
Troponin I / analysis
Troponin I / blood*

Substances

Aluminum Compounds
Antibodies, Immobilized
Biomarkers
Troponin I
aluminum gallium nitride
gallium nitride
Gallium