Multi-locus sequence typing (MLST) of non-fermentative Gram-negative bacilli isolated from bloodstream infections in southern Poland

Folia Microbiol (Praha). 2018 Mar;63(2):191-196. doi: 10.1007/s12223-017-0550-7. Epub 2017 Sep 22.

Abstract

Non-fermentative Gram-negative bacilli are now one of the most important causes of severe infections in Polish hospitals. Acinetobacter species are serious concern because of the high prevalence of multi-drug resistance among strains. Resistance profiles for 53 Gram-negative non-fermentative blood isolates were done. MLST was carried out using 44 strains representing the most commonly isolated species: A. baumannii, P. aeruginosa, and S. maltophilia. MLST revealed that all 22 A. baumannii belonged to sequence type (ST) 2. The P. aeruginosa isolates belonged to 10 different STs. Four S. maltophilia isolates matched STs present in the database (ST4, ST15, ST116, ST142), seven isolates showing novel sequence types. Among P. aeruginosa and S. maltophilia PFGE confirmed the genetical variety of strains.

Keywords: Bloodstream infection; MLST; Non-fermentative gram negative bacilli.

MeSH terms

  • Aged
  • Anti-Bacterial Agents / pharmacology
  • Bacteremia / microbiology*
  • Cross Infection / blood
  • Cross Infection / microbiology
  • Female
  • Gram-Negative Bacteria / classification
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / isolation & purification*
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Multilocus Sequence Typing
  • Phylogeny
  • Poland

Substances

  • Anti-Bacterial Agents