Abstract
A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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CRISPR-Cas Systems / genetics
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Cell Line, Tumor
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Color
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Cytoskeleton / metabolism*
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Fluorescence
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Fluorescent Dyes / chemical synthesis
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacology*
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Fluorescent Dyes / radiation effects
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Gene Editing
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Humans
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Infrared Rays
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Microscopy, Confocal
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Microscopy, Fluorescence / methods*
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Organosilicon Compounds / chemical synthesis
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Organosilicon Compounds / chemistry
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Organosilicon Compounds / pharmacology*
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Organosilicon Compounds / radiation effects
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Quinolizines / chemical synthesis
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Quinolizines / chemistry
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Quinolizines / pharmacology*
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Quinolizines / radiation effects
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Recombinant Fusion Proteins / genetics
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Vimentin / genetics
Substances
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Fluorescent Dyes
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Organosilicon Compounds
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Quinolizines
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Recombinant Fusion Proteins
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Vimentin