Abstract
In order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain Chemistry
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Female
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Intermediate Filament Proteins / biosynthesis
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Intermediate Filament Proteins / genetics*
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Microinjections
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Neurofilament Proteins
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Poly A / genetics*
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Protein Biosynthesis
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RNA, Messenger / genetics*
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Rats
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Receptors, Serotonin / biosynthesis
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Receptors, Serotonin / genetics
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Xenopus laevis
Substances
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Intermediate Filament Proteins
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Neurofilament Proteins
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RNA, Messenger
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Receptors, Serotonin
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Poly A