Biosynthesis of the neurofilament heavy subunit in Xenopus oocytes microinjected with rat brain poly(A)+ RNA

Mol Biol Rep. 1987;12(4):265-71. doi: 10.1007/BF00444678.

Abstract

In order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry
  • Female
  • Intermediate Filament Proteins / biosynthesis
  • Intermediate Filament Proteins / genetics*
  • Microinjections
  • Neurofilament Proteins
  • Poly A / genetics*
  • Protein Biosynthesis
  • RNA, Messenger / genetics*
  • Rats
  • Receptors, Serotonin / biosynthesis
  • Receptors, Serotonin / genetics
  • Xenopus laevis

Substances

  • Intermediate Filament Proteins
  • Neurofilament Proteins
  • RNA, Messenger
  • Receptors, Serotonin
  • Poly A