Abstract
The endophytic Trichoderma atroviridae UB-LMA was isolated as a symbiont of Taxus baccata and analyzed for its antimicrobial activity. By applying an original approach consisting of solid-state cultivation coupled with solid-phase extraction, a new methyl (R,E)-3-(1-hydroxy-4-oxocyclopent-2-en-1-yl)-acrylate derivative named EA-2801 (1) was isolated together with the previously reported isonitrin A and dermadin methyl ester. The chemical structure of 1 was determined by NMR and MS. Compound 1 showed antimicrobial activity against a panel of Gram-positive and -negative bacteria.
MeSH terms
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Acrylates / chemistry
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Acrylates / isolation & purification
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Acrylates / pharmacology*
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology*
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Cyclopentanes / chemistry
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Cyclopentanes / isolation & purification
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Cyclopentanes / pharmacology*
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Gram-Negative Bacteria / drug effects*
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Gram-Positive Bacteria / drug effects*
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Heterocyclic Compounds, 2-Ring / chemistry
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Heterocyclic Compounds, 2-Ring / isolation & purification
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Heterocyclic Compounds, 2-Ring / pharmacology*
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Nitriles / chemistry
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Nitriles / isolation & purification
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Nitriles / pharmacology*
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Solid Phase Extraction
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Spiro Compounds / chemistry
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Spiro Compounds / isolation & purification
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Spiro Compounds / pharmacology*
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Trichoderma / chemistry*
Substances
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Acrylates
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Anti-Bacterial Agents
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Cyclopentanes
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Heterocyclic Compounds, 2-Ring
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Nitriles
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Spiro Compounds
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dermadin methyl ester
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isonitrin A
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methyl (R,E)-3-(1-hydroxy-4-oxocyclopent-2-en-1-yl)-acrylate