In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT

Brian J Beliveau; Alistair N Boettiger; Guy Nir; Bogdan Bintu; Peng Yin; Xiaowei Zhuang; C-Ting Wu

doi:10.1007/978-1-4939-7265-4_19

In Situ Super-Resolution Imaging of Genomic DNA with OligoSTORM and OligoDNA-PAINT

Methods Mol Biol. 2017:1663:231-252. doi: 10.1007/978-1-4939-7265-4_19.

Authors

Brian J Beliveau^{1

2

3}, Alistair N Boettiger^{4

5

6}, Guy Nir¹, Bogdan Bintu^{4

7}, Peng Yin^{8

9}, Xiaowei Zhuang^{10

11

12}, C-Ting Wu¹³

Affiliations

¹ Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA.
² Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
³ Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA.
⁴ Howard Hughes Medical Institute, Cambridge, MA, 02138, USA.
⁵ Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, USA.
⁶ Department of Developmental Biology, Stanford University, Stanford, CA, 94305, USA.
⁷ Department of Physics, Harvard University, Cambridge, MA, 02138, USA.
⁸ Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA. py@hms.harvard.edu.
⁹ Department of Systems Biology, Harvard Medical School, Boston, MA, 02115, USA. py@hms.harvard.edu.
¹⁰ Howard Hughes Medical Institute, Cambridge, MA, 02138, USA. zhuang@chemistry.harvard.edu.
¹¹ Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, USA. zhuang@chemistry.harvard.edu.
¹² Department of Physics, Harvard University, Cambridge, MA, 02138, USA. zhuang@chemistry.harvard.edu.
¹³ Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA, 02115, USA. twu@genetics.med.harvard.edu.

Abstract

OligoSTORM and OligoDNA-PAINT meld the Oligopaint technology for fluorescent in situ hybridization (FISH) with, respectively, Stochastic Optical Reconstruction Microscopy (STORM) and DNA-based Point Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) to enable in situ single-molecule super-resolution imaging of nucleic acids. Both strategies enable ≤20 nm resolution and are appropriate for imaging nanoscale features of the genomes of a wide range of species, including human, mouse, and fruit fly (Drosophila).

Keywords: Chromatin; Chromosomes; DNA-PAINT; FISH; Genome; OligoDNA-PAINT; OligoSTORM; Oligopaint; STORM; Single-molecule; Super-resolution.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA / chemistry*
Drosophila
Genome
Humans
In Situ Hybridization, Fluorescence / methods*
Mice
Single Molecule Imaging / methods*

Substances

DNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding