Colorimetric detection of D-dimer in a paper-based immunodetection device

Anal Biochem. 2017 Dec 1:538:5-12. doi: 10.1016/j.ab.2017.09.009. Epub 2017 Sep 15.

Abstract

A microfluidic paper-based analytical device (μPADs) immunoassay for detection of the blood native biomarker D-dimer is reported. The μPAD is created by wax printing on a single piece of chromatographic paper and combined with an anti-D-dimer capture antibody and conjugates of anti-D-dimer antibody with 40 nm gold nanoparticles. The presence of D-dimer in buffer/simulated plasma samples is successfully reported for concentrations as low as 15 ng D-dimer/mL. Linearity between signal intensity and D-dimer concentration is observed up to 100 ng/mL. Using an appropriate dilution, the test could be used to yield positive results only for those samples with a D-dimer concentration above the clinically relevant threshold range of 250-500 ng/mL. Finally, the merits and pitfalls of using μPADs as compared to lateral flow devices in immunoassays are discussed.

Keywords: Antibody; D-dimer; Gold nanoparticles; Immunoassay; Paper microfluidics; μPAD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / immunology
  • Biomarkers / blood
  • Colorimetry / instrumentation*
  • Fibrin Fibrinogen Degradation Products / analysis*
  • Fibrin Fibrinogen Degradation Products / immunology
  • Gold / chemistry
  • Humans
  • Immunoassay / instrumentation*
  • Immunoassay / methods*
  • Metal Nanoparticles / chemistry
  • Microfluidic Analytical Techniques / instrumentation
  • Paper*

Substances

  • Antibodies
  • Biomarkers
  • Fibrin Fibrinogen Degradation Products
  • fibrin fragment D
  • Gold