Flavobacterium chuncheonense sp. nov. and Flavobacterium luteum sp. nov., isolated from a freshwater lake

Abstract

Two Gram-staining-negative, orange coloured, non-motile, rod-shaped bacterial strains, designated strains IMCC26013^T and IMCC26026^T, were isolated from a freshwater sample collected from Lake Soyang in Korea. The 16S rRNA gene-based phylogenetic analyses showed that both strains belonged to the genus Flavobacterium and that strains IMCC26013^T and IMCC26026^T were most closely related to Flavobacterium psychrophilum (96.5 %) and Flavobacterium myungsuense (97.7 %), respectively. DNA G+C contents of strains IMCC26013^T and IMCC26026^T were 37.8 and 33.7 mol%, respectively. DNA-DNA relatedness between strain IMCC26026^T and F. myungsuense HMD1033^T was 56.4 %, showing a novel species status of strain IMCC26026^T. Major fatty acid constituents (>10 %) of strain IMCC26013^T were iso-C15 : 1 G, C15 : 1 ω6c, C17 : 1 ω6c and summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and those of strain IMCC26026^T were iso-C15 : 0, anteiso-C15 : 0 and summed feature 3. Respiratory quinone detected in the two strains was MK-6. Both strains contained phosphatidylethanolamine as a major polar lipid. On the basis of these results, strains IMCC26013^T and IMCC26026^T were considered to represent novel species in the genus Flavobacterium, for which the names Flavobacterium chuncheonense (type strain IMCC26013^T=KCTC 52573^T=NBRC 112526^T), and Flavobacterium luteum (type strain IMCC26026^T=KCTC 52572^T=NBRC 112527^T) are proposed, respectively.

Keywords: Flavobacterium; freshwater; new species; taxonomy.