Engineering Non-transgenic Gynoecious Cucumber Using an Improved Transformation Protocol and Optimized CRISPR/Cas9 System

Bowen Hu; Dawei Li; Xin Liu; Jingjing Qi; Dongli Gao; Shuqiao Zhao; Sanwen Huang; Jinjing Sun; Li Yang

doi:10.1016/j.molp.2017.09.005

Engineering Non-transgenic Gynoecious Cucumber Using an Improved Transformation Protocol and Optimized CRISPR/Cas9 System

Mol Plant. 2017 Dec 4;10(12):1575-1578. doi: 10.1016/j.molp.2017.09.005. Epub 2017 Sep 15.

Authors

Bowen Hu¹, Dawei Li², Xin Liu³, Jingjing Qi⁴, Dongli Gao⁵, Shuqiao Zhao⁶, Sanwen Huang⁵, Jinjing Sun⁷, Li Yang⁸

Affiliations

¹ Key Laboratory of Genome Analysis of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China; Gembloux Agro-Bio Tech, University of Liege, Liege 4000, Belgium.
² College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
³ Key Laboratory of Genome Analysis of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
⁴ College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
⁵ Key Laboratory of Genome Analysis of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
⁶ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
⁷ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: sunjinjing@caas.cn.
⁸ Key Laboratory of Genome Analysis of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: yangli_solab@163.com.

PMID: 28919533
DOI: 10.1016/j.molp.2017.09.005

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems / genetics*
Cucumis sativus / genetics*
Genetic Engineering / methods*
Transformation, Genetic*