Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection

Litao Yang; Sheng Quan; Dabing Zhang

doi:10.1007/978-1-4939-7337-8_16

Endogenous Reference Genes and Their Quantitative Real-Time PCR Assays for Genetically Modified Bread Wheat (Triticum aestivum L.) Detection

Methods Mol Biol. 2017:1679:259-268. doi: 10.1007/978-1-4939-7337-8_16.

Authors

Litao Yang¹, Sheng Quan¹, Dabing Zhang²

Affiliations

¹ National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-Bor Luh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
² National Center for Molecular Characterization of Genetically Modified Organisms, SJTU-Bor Luh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. zhangdb@sjtu.edu.cn.

PMID: 28913806
DOI: 10.1007/978-1-4939-7337-8_16

Abstract

Endogenous reference genes (ERG) and their derivate analytical methods are standard requirements for analysis of genetically modified organisms (GMOs). Development and validation of suitable ERGs is the primary step for establishing assays that monitoring the genetically modified (GM) contents in food/feed samples. Herein, we give a review of the ERGs currently used for GM wheat analysis, such as ACC1, PKABA1, ALMT1, and Waxy-D1, as well as their performances in GM wheat analysis. Also, we discussed one model for developing and validating one ideal RG for one plant species based on our previous research work.

Keywords: Endogenous reference gene; GMO detection; Genetically modified wheat.

Publication types

Review

MeSH terms

Genes, Plant*
Plants, Genetically Modified*
Real-Time Polymerase Chain Reaction* / methods
Reference Standards*
Reproducibility of Results
Triticum / genetics*