[Letter to the Editor] Incorrect assignment of affected nucleotides in footprinting/probing experiments

Biotechniques. 2017 Sep 1;63(3):105-106. doi: 10.2144/000114585.

Abstract

Address correspondence to Sergey Belikov or Lars Wieslander, Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91 Stockholm, Sweden. E-mail: sergey.belikov@su.se or lars.wieslander@su.se.

Keywords: Sanger sequencing; dITP; primer extension.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Footprinting*
  • DNA Primers / chemistry*
  • DNA Probes / chemistry*
  • DNA Probes / genetics
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • Deoxyguanine Nucleotides / metabolism
  • Inosine Triphosphate / analogs & derivatives
  • Inosine Triphosphate / metabolism
  • RNA, Ribosomal, 18S / chemistry
  • RNA, Ribosomal, 18S / genetics
  • Reverse Transcription
  • Sequence Analysis, DNA

Substances

  • DNA Primers
  • DNA Probes
  • DNA-Binding Proteins
  • Deoxyguanine Nucleotides
  • RNA, Ribosomal, 18S
  • Inosine Triphosphate
  • 2'-deoxyinosine triphosphate
  • deoxyguanosine triphosphate