The use of dissolvable layered double hydroxide components in an in situ solid-phase extraction for chromatographic determination of tetracyclines in water and milk samples

Nattaphorn Phiroonsoontorn; Sira Sansuk; Yanawath Santaladchaiyakit; Supalax Srijaranai

doi:10.1016/j.chroma.2017.09.005

The use of dissolvable layered double hydroxide components in an in situ solid-phase extraction for chromatographic determination of tetracyclines in water and milk samples

J Chromatogr A. 2017 Oct 13:1519:38-44. doi: 10.1016/j.chroma.2017.09.005. Epub 2017 Sep 4.

Authors

Nattaphorn Phiroonsoontorn¹, Sira Sansuk¹, Yanawath Santaladchaiyakit², Supalax Srijaranai³

Affiliations

¹ Materials Chemistry Research Center, Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand.
² Department of Chemistry, Faculty of Engineering, Rajamangala University of Technology Isan, Khon Kaen, 40000, Thailand.
³ Materials Chemistry Research Center, Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand. Electronic address: supalax@kku.ac.th.

PMID: 28899552
DOI: 10.1016/j.chroma.2017.09.005

Abstract

This research presents a simple and green in situ solid phase extraction (is-SPE) combined with high-performance liquid chromatography (HPLC) for the simultaneous analysis of tetracyclines (TCs) including tetracycline, oxytetracycline, and chlortetracycline. In is-SPE, TCs were efficiently extracted through the precipitation formation of dissolvable layered double hydroxides (LDHs) by mixing the LDH components such as magnesium and aluminum ions (both in metal chloride salts) thoroughly in an alkaline sample solution. After the centrifugation, the precipitate was completely dissolved with trifluoroacetic acid to release the enriched TCs, and then analyzed by HPLC. Under optimized conditions, this method gave good enrichment factors (EFs) of 41-93 with low limits of detection (LODs) of 0.7-6μg/L and limits of quantitation (LOQs) of 3-15μg/L. Also, the proposed method was successfully applied for the determination of TCs in water and milk samples with the recoveries ranging from 81.7-108.1% for water and 55.7-88.7% for milk.

Keywords: High performance liquid chromatography; Layered double hydroxides; Milk; Solid phase extraction; Tetracyclines; Water.

MeSH terms

Animals
Anti-Bacterial Agents / analysis
Chromatography, High Pressure Liquid*
Food Analysis / methods*
Hydroxides / chemistry
Limit of Detection
Milk / chemistry*
Solid Phase Extraction / instrumentation*
Tetracyclines / analysis*
Water / chemistry*

Substances

Anti-Bacterial Agents
Hydroxides
Tetracyclines
Water