Historic data on biodiversity provide the context for present observations and allow studying long-term changes in marine populations. Here we present multiple datasets on fish and fisheries of the Adriatic Sea covering the last two centuries encompassing from qualitative observations to standardised scientific monitoring. The datasets consist of three groups: (1) early naturalists' descriptions of fish fauna, including information (e.g., presence, perceived abundance, size) on 255 fish species for the period 1818-1936; (2) historical landings from major Northern Adriatic fish markets (Venice, Trieste, Rijeka) for the period 1902-1968, Italian official landings for the Northern and Central Adriatic (1953-2012) and landings from the Lagoon of Venice (1945-2001); (3) trawl-survey data from seven surveys spanning the period 1948-1991 and including Catch per Unit of Effort data (kgh-1 and/or nh-1) for 956 hauls performed at 301 stations. The integration of these datasets has already demonstrated to be useful to analyse historical marine community changes over time, and its availability through open-source data portal will facilitate analyses in the framework of marine historical ecology.