Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection

Mo-Yuan Shen; Jie-Fu Chen; Chun-Hao Luo; Sangjun Lee; Cheng-Hsuan Li; Yung-Ling Yang; Yu-Han Tsai; Bo-Cheng Ho; Li-Rong Bao; Tien-Jung Lee; Yu Jen Jan; Ya-Zhen Zhu; Shirley Cheng; Felix Y Feng; Peilin Chen; Shuang Hou; Vatche Agopian; Yu-Sheng Hsiao; Hsian-Rong Tseng; Edwin M Posadas; Hsiao-Hua Yu

doi:10.1002/adhm.201700701

Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection

Adv Healthc Mater. 2018 Feb;7(3):10.1002/adhm.201700701. doi: 10.1002/adhm.201700701. Epub 2017 Sep 11.

Authors

Mo-Yuan Shen¹, Jie-Fu Chen², Chun-Hao Luo¹, Sangjun Lee³, Cheng-Hsuan Li¹, Yung-Ling Yang¹, Yu-Han Tsai¹, Bo-Cheng Ho⁴, Li-Rong Bao³, Tien-Jung Lee³, Yu Jen Jan³, Ya-Zhen Zhu³, Shirley Cheng², Felix Y Feng⁵, Peilin Chen⁶, Shuang Hou⁷, Vatche Agopian⁷, Yu-Sheng Hsiao⁴, Hsian-Rong Tseng³, Edwin M Posadas², Hsiao-Hua Yu¹

Affiliations

¹ Smart Organic Material Laboratory, Institute of Chemistry, Academia Sinica, No. 128, Sec. 2, Academia Rd., Nankang, Taipei, 11529, Taiwan.
² Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Los Angeles, CA, 90048, USA.
³ Department of Molecular and Medical Pharmacology, California NanoSystems Institute, University of California, Los Angeles, 570 Westwood Plaza, Los Angeles, CA, 90095-1770, USA.
⁴ Department of Material Engineering, Ming Chi University of Technology, 84 Gungjuan Rd., Taishan Dist., New Taipei City, 24301, Taiwan.
⁵ Departments of Radiation Oncology, Urology, and Medicine, University of California, San Francisco, San Francisco, CA, 94158, USA.
⁶ Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
⁷ Liver Transplantation and Hepatobiliary Surgery, Department of Surgery, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA.

Abstract

A glycan-stimulated and poly(3,4-ethylene-dioxythiophene)s (PEDOT)-based nanomaterial platform is fabricated to purify circulating tumor cells (CTCs) from blood samples of prostate cancer (PCa) patients. This new platform, phenylboronic acid (PBA)-grafted PEDOT NanoVelcro, combines the 3D PEDOT nanosubstrate, which greatly enhances CTC capturing efficiency, with a poly(EDOT-PBA-co-EDOT-EG3) interfacial layer, which not only provides high specificity for CTC capture upon antibody conjugation but also enables competitive binding of sorbitol to gently release the captured cells. CTCs purified by this PEDOT NanoVelcro chip provide well-preserved RNA transcripts for the analysis of the expression level of several PCa-specific RNA biomarkers, which may provide clinical insights into the disease.

Keywords: RNA biomarkers; circulating tumor cells; poly(3,4-ethylene-dioxythiophene)s; prostate cancer; responsive materials.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / analysis*
Bridged Bicyclo Compounds, Heterocyclic / chemistry*
Cell Line, Tumor
Humans
Male
Nanostructures / chemistry*
Neoplastic Cells, Circulating / metabolism
Neoplastic Cells, Circulating / pathology*
Polymers / chemistry*
Prostatic Neoplasms / metabolism
Prostatic Neoplasms / pathology*
RNA / analysis*

Substances

Biomarkers
Bridged Bicyclo Compounds, Heterocyclic
Polymers
poly(3,4-ethylene dioxythiophene)
RNA

Abstract

Publication types

MeSH terms

Substances

Grants and funding