Characterization of a novel mosquitocidal toxin of Cry50Ba and its potential synergism with other mosquitocidal toxins

Toxicon. 2017 Nov:138:165-168. doi: 10.1016/j.toxicon.2017.08.025. Epub 2017 Sep 7.

Abstract

A putative toxin gene of cry50Ba was successfully expressed in E. coli cells and confirmed that the purified Cry50Ba toxin had very high toxic activity against Culex quinquefasciatus larvae. Furthermore, the potential synergism of Cry50Ba toxin with Cry2Aa, Cry4Aa and Cry11Aa at a ratio of 1:1 was investigated. Although no significant synergism with other toxins was observed, the Cry50Ba as a novel toxin could be used to delay rapid onset of resistance in mosquito.

Keywords: Bacillus thuringiensis; Cry50Ba; Culex quinquefasciatus; Synergism.

MeSH terms

  • Animals
  • Bacillus thuringiensis / chemistry
  • Bacillus thuringiensis / genetics*
  • Bacterial Toxins / genetics
  • Bacterial Toxins / pharmacology*
  • Culex / drug effects*
  • Escherichia coli / genetics
  • Insecticide Resistance
  • Larva / drug effects
  • Organisms, Genetically Modified
  • Recombinant Proteins

Substances

  • Bacterial Toxins
  • Recombinant Proteins