Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures

Penelope J Adamson; Mahmood A Al Kindi; Jing J Wang; Alex D Colella; Timothy K Chataway; Nikolai Petrovsky; Tom P Gordon; David L Gordon

doi:10.1016/j.vaccine.2017.08.053

Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures

Vaccine. 2017 Oct 9;35(42):5576-5580. doi: 10.1016/j.vaccine.2017.08.053. Epub 2017 Sep 6.

Authors

Penelope J Adamson¹, Mahmood A Al Kindi², Jing J Wang³, Alex D Colella⁴, Timothy K Chataway⁵, Nikolai Petrovsky⁶, Tom P Gordon⁷, David L Gordon⁸

Affiliations

¹ Department of Microbiology and Infectious Diseases, Flinders University and SA Pathology, Flinders Medical Centre, Bedford Park, SA 5042, Australia. Electronic address: penelope.adamson@sa.gov.au.
² Department of Immunology, Flinders University and SA Pathology, Flinders Medical Centre, Bedford Park, SA 5042, Australia. Electronic address: dralkindi@squ.edu.om.
³ Department of Immunology, Flinders University and SA Pathology, Flinders Medical Centre, Bedford Park, SA 5042, Australia. Electronic address: jing.wang@flinders.edu.au.
⁴ Flinders Proteomic Facility, Flinders University, Flinders Medical Centre, Bedford Park, SA 5042, Australia. Electronic address: Alexander.Colella@sa.gov.au.
⁵ Flinders Proteomic Facility, Flinders University, Flinders Medical Centre, Bedford Park, SA 5042, Australia. Electronic address: tim.chataway@flinders.edu.au.
⁶ Department of Endocrinology, Flinders University and Vaxine Pty Ltd., Flinders Medical Centre, Bedford Park, SA 5042, Australia. Electronic address: nikolai.petrovsky@flinders.edu.au.
⁷ Department of Immunology, Flinders University and SA Pathology, Flinders Medical Centre, Bedford Park, SA 5042, Australia. Electronic address: t.gordon@flinders.edu.au.
⁸ Department of Microbiology and Infectious Diseases, Flinders University and SA Pathology, Flinders Medical Centre, Bedford Park, SA 5042, Australia. Electronic address: d.gordon@flinders.edu.au.

PMID: 28888340
DOI: 10.1016/j.vaccine.2017.08.053

Abstract

Analysis of the anti-haemagglutinin serum antibody proteome from six H1N1pdm09 influenza A vaccinated subjects demonstrated restricted IgG1 heavy chain species encoded by IGHV5-51 and IGHV3-7 gene families in 2 subjects and either IGHV5-51 or IGHV3-7 in 4 individuals. All subjects exhibited a dominant IGKV3-20 light chain, however 5 subjects also exhibited IGKV3-11 and IGKV4-1 families. Sequences were closely aligned with the matched germline sequence, with few shared mutations. This study illustrates the feasibility of using a proteomic approach to determine the expressed V region signatures of serum antibodies induced by vaccination.

Keywords: Influenza A; Mass spectrometric sequencing; Proteomics; Serum antibody repertoire; Vaccination.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Aged
Amino Acid Sequence
Antibodies, Viral / immunology*
Female
Hemagglutinins / immunology*
Humans
Immunoglobulin Variable Region / immunology*
Influenza, Human / immunology*
Influenza, Human / prevention & control*
Male
Middle Aged
Proteome / immunology*
Proteomics / methods
Vaccination / methods

Substances

Antibodies, Viral
Hemagglutinins
Immunoglobulin Variable Region
Proteome