Synergistic degradation of pyrene by five culturable bacteria in a mangrove sediment-derived bacterial consortium

Pagakrong Wanapaisan; Natthariga Laothamteep; Felipe Vejarano; Joydeep Chakraborty; Masaki Shintani; Chanokporn Muangchinda; Tomomi Morita; Chiho Suzuki-Minakuchi; Kengo Inoue; Hideaki Nojiri; Onruthai Pinyakong

doi:10.1016/j.jhazmat.2017.08.062

Synergistic degradation of pyrene by five culturable bacteria in a mangrove sediment-derived bacterial consortium

J Hazard Mater. 2018 Jan 15:342:561-570. doi: 10.1016/j.jhazmat.2017.08.062. Epub 2017 Sep 19.

Affiliations

¹ Department of Microbiology, Faculty of Science, Chulalongkorn University, 254 Phyathai Road, Pathumwan, Bangkok 10330, Thailand.
² Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
³ Faculty of Engineering, Shizuoka University, 3-5-1 Johoku, Naka-ku, Hamamatsu, Shizuoka 432-8561, Japan.
⁴ Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Department of Bioscience and Engineering, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama, Saitama 337-8570, Japan.
⁵ Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki, Miyazaki 889-2192, Japan.
⁶ Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan. Electronic address: anojiri@mail.ecc.u-tokyo.ac.jp.
⁷ Department of Microbiology, Faculty of Science, Chulalongkorn University, 254 Phyathai Road, Pathumwan, Bangkok 10330, Thailand; Research Program on Remediation Technologies for Petroleum Contamination, Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, 254 Phyathai Road, Pathumwan, Bangkok 10330, Thailand; Omics Sciences and Bioinformatics Center, Faculty of Science, Chulalongkorn University, 254 Phyathai Road, Pathumwan, Bangkok 10330, Thailand. Electronic address: onruthai@gmail.com.

PMID: 28886568
DOI: 10.1016/j.jhazmat.2017.08.062

Abstract

A pyrene-degrading microbial consortium was obtained after enrichment with mangrove sediment collected from Thailand. Five cultivable bacteria (Mycobacterium spp. PO1 and PO2, Novosphingobium pentaromativorans PY1, Ochrobactrum sp. PW1, and Bacillus sp. FW1) were successfully isolated from the consortium. Draft genomes of them showed that two different morphotypes of Mycobacterium (PO1 and PO2), possessed a complete gene set for pyrene degradation. PY1 contained genes for phthalate assimilation via protocatechuate, a central intermediate, by meta-cleavage pathway, and PW1 possessed genes for protocatechuate degradation via ortho-cleavage pathway. The occurrence of biosurfactant-producing genes in FW1 suggests the involvement in enhancing the pyrene bioavailability. Biotransformation experiments revealed that Mycobacterium completely degraded 100mgL^-1 pyrene within six days, whereas no significant degradation was observed with the others. Notably, PY1 and PW1 exhibited higher activity for protocatechuate degradation than the others. The artificially reconstructed consortia containing Mycobacterium with the other three strains (PY1, PW1 and FW1) showed three-fold higher degradation rate for pyrene than the individual Mycobacterium. The enhanced pyrene biodegradation achieved in the consortium was due to the cooperative interaction of bacterial mixture. Our findings showing that synergistic degradation of pyrene in the consortium will facilitate the application of the defined bacterial consortium in bioremediation.

Keywords: Mangrove sediment; Mycobacterium; Novosphingobium; Pyrene; Synergistic degradation.

MeSH terms

Biodegradation, Environmental
Geologic Sediments
Mycobacterium / chemistry
Mycobacterium / metabolism*
Pyrenes / chemistry
Pyrenes / metabolism*

Substances

Pyrenes
pyrene