Expression and isolation of recombinant tau

Rebecca L Best; Peter J Chung; Sarah J Benbow; April Savage; Nichole E LaPointe; Cyrus R Safinya; Stuart C Feinstein

doi:10.1016/bs.mcb.2017.06.001

Expression and isolation of recombinant tau

Methods Cell Biol. 2017:141:3-26. doi: 10.1016/bs.mcb.2017.06.001. Epub 2017 Jul 24.

Authors

Rebecca L Best¹, Peter J Chung², Sarah J Benbow¹, April Savage³, Nichole E LaPointe⁴, Cyrus R Safinya², Stuart C Feinstein⁵

Affiliations

¹ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; University of California, Santa Barbara, Santa Barbara, CA, United States.
² University of California, Santa Barbara, Santa Barbara, CA, United States.
³ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; College of Creative Studies, University of California, Santa Barbara, Santa Barbara, CA, United States.
⁴ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States.
⁵ Neuroscience Research Institute, University of California, Santa Barbara, Santa Barbara, CA, United States; University of California, Santa Barbara, Santa Barbara, CA, United States; College of Creative Studies, University of California, Santa Barbara, Santa Barbara, CA, United States. Electronic address: feinstei@lifesci.ucsb.edu.

PMID: 28882309
DOI: 10.1016/bs.mcb.2017.06.001

Abstract

In this chapter, we describe methods for the purification of both untagged and polyhistidine-tagged tau protein. These protocols utilize a bacterial expression system to produce the tau isoform of interest, followed by heat treatment and column chromatography to separate tau from impurities. These techniques yield a biochemically pure protein with which to pursue any number of questions regarding the mechanisms of tau action.

Keywords: Chromatography; Recombinant tau; Tau expression; Tau isolation; Tau purification.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Affinity / methods*
Histidine / chemistry
Histidine / genetics
Histidine / metabolism*
Humans
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
tau Proteins / genetics
tau Proteins / isolation & purification*
tau Proteins / metabolism*

Substances

MAPT protein, human
Recombinant Proteins
tau Proteins
polyhistidine
Histidine

Grants and funding

R01 NS035010/NS/NINDS NIH HHS/United States