Data on the construction of a recombinant HEK293 cell line overexpressing hERG potassium channel and examining the presence of hERG mRNA and protein expression

Yi Fan Teah; Muhammad Asyraf Abduraman; Azimah Amanah; Mohd Ilham Adenan; Shaida Fariza Sulaiman; Mei Lan Tan

doi:10.1016/j.dib.2017.08.008

Data on the construction of a recombinant HEK293 cell line overexpressing hERG potassium channel and examining the presence of hERG mRNA and protein expression

Data Brief. 2017 Aug 12:14:584-591. doi: 10.1016/j.dib.2017.08.008. eCollection 2017 Oct.

Authors

Yi Fan Teah¹, Muhammad Asyraf Abduraman², Azimah Amanah¹, Mohd Ilham Adenan³, Shaida Fariza Sulaiman⁴, Mei Lan Tan^{1

2}

Affiliations

¹ Malaysian Institute of Pharmaceuticals & Nutraceuticals, National Institutes of Biotechnology Malaysia (NIBM), Ministry of Science, Technology and Innovation Malaysia, Pulau Pinang, Malaysia.
² Advanced Medical & Dental Institute, Universiti Sains Malaysia, Pulau Pinang, Malaysia.
³ Atta-ur-Rahman Institute for Natural Product Discovery, Universiti Teknologi MARA (UiTM), Selangor Darul Ehsan, Malaysia.
⁴ School of Biological Sciences, Universiti Sains Malaysia, Pulau Pinang, Malaysia.

Abstract

The data presented in this article are related to the research article entitled "The effects of deoxyelephantopin on the cardiac delayed rectifier potassium channel current (I_Kr) and human ether-a-go-go-related gene (hERG) expression" (Y.F. Teah, M.A. Abduraman, A. Amanah, M.I. Adenan, S.F. Sulaiman, M.L. Tan) [1], which the possible hERG blocking properties of deoxyelephantopin were investigated. This article describes the construction of human embryonic kidney 293 (HEK293) cells overexpressing HERG potassium channel and verification of the presence of hERG mRNA and protein expression in this recombinant cell line.

Keywords: Recombinant cell line; hERG; hERG mRNA expression; hERG protein expression.