In-line balanced detection stimulated Raman scattering microscopy

Sci Rep. 2017 Sep 6;7(1):10745. doi: 10.1038/s41598-017-09839-1.

Abstract

We introduce a novel configuration for stimulated Raman scattering (SRS) microscopy, called In-line Balanced Detection (IBD), which employs a birefringent plate to generate a time-delayed polarization-multiplexed collinear replica of the probe, acting as a reference. Probe and reference cross the sample at the same position, thus maintaining their balance during image acquisition. IBD can be implemented in any conventional SRS setup, by adding a few simple elements, bringing its sensitivity close to the shot-noise limit even with a noisy laser. We tested IBD with a fiber-format laser system and observed signal-to-noise ratio improvement by up to 30 dB.

Publication types

  • Research Support, Non-U.S. Gov't