Structural Analysis of Glycans

Barbara Mulloy; Anne Dell; Pamela Stanley; James H. Prestegard

doi:10.1101/glycobiology.3e.050

Structural Analysis of Glycans

Review

In: Essentials of Glycobiology [Internet]. 3rd edition. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2015. Chapter 50.

2017.

Authors

Barbara Mulloy, Anne Dell, Pamela Stanley, James H. Prestegard

Book Editors

Book Affiliations

¹ Distinguished Professor of Medicine and Cellular & Molecular Medicine, Co-Director, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA
² Professor of Surgery, Director, National Center for Functional Glycomics, Harvard Medical School, Cambridge, Massachusetts, USA
³ Distinguished Professor of Cellular & Molecular Medicine, Co-Director, Glycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA
⁴ Horace W. Goldsmith Foundation Chair and Professor of Cell Biology, Albert Einstein College of Medicine, New York, New York, USA
⁵ Professor and Chairman of Biological Chemistry, Johns Hopkins University, Baltimore, Maryland, USA
⁶ Professor of Mycology, ETH Zürich, Zürich, Switzerland
⁷ Regents Professor and Director, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia, USA
⁸ Professor, Laboratory of Immunoglycobiology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
⁹ Professor of Glycoproteomics and Glycomics, Macquarie University and Institute for Glycomics, Griffith University, Sydney, Australia
¹⁰ Professor and Eminent Scholar, University of Georgia, Athens, Georgia, USA
¹¹ Professor, Departments of Pharmacology and Neuroscience, Johns Hopkins University, Baltimore, Maryland, USA
¹² Director, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany

Excerpt

This chapter surveys methods for the structural characterization of glycans, including residue composition, linkage types, and attachment to aglycones. It covers methods for detection of specific glycan sequences on glycoproteins and cellular surfaces, and it covers methods for characterizing structures in three dimensions. The methods vary widely, ranging from classical chemical methods for detection and characterization of glycans on isolated products, to sensitive fluorescence methods used in combination with glycan-binding proteins and microscopy on cells and tissues. Methods also include nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry (MS) approaches that allow more detailed structural characterization.

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