Semi-preparative high-performance countercurrent chromatography method for the purification of chemically synthesized ATP analogue, ApppI

Abstract

An efficient high-performance countercurrent chromatography (HPCCC) based method has been developed for the purification of chemically synthesized 1-adenosin-5'-yl 3-(3-methylbut-3-enyl)triphosphoric acid diester (ApppI). ApppI is an adenosine triphosphate (ATP) analogue with biological significance due to its varied actions in the body. ApppI was synthesized and purified as its tetrabutylammonium (TBA) salt and converted successfully into its more practical sodium salt form after purification. The amount of TBA hydroxide (2.0, 2.5 and 3.0 eq) used in the synthesis of ApppI was shown to exert an effect on the purification process with HPCCC and on the overall yield (8%, 16% and 22%, respectively). 1-Adenosin-5'-yl 3-(3-methylbut-3-enyl)diphosphoric acid diester (AppI) was also isolated as a side product.

Keywords: 1-adenosin-5′ -yl 3-(3-methylbut-3-enyl)triphosphoric acid diester; 1-adenosin-5′-yl 3-(3-methylbut-3-enyl)diphosphoric acid diester; ATP; High-performance countercurrent chromatography.