High-Density Mapping of Brain Slices using a Large Multi-Functional High-Density CMOS Microelectrode Array System

Vijay Viswam; Raziyeh Bounik; Amir Shadmani; Jelena Dragas; Marie Obien; Jan Müller; Yihui Chen; Andreas Hierlemann

doi:10.1109/TRANSDUCERS.2017.7994006

High-Density Mapping of Brain Slices using a Large Multi-Functional High-Density CMOS Microelectrode Array System

Int Solid State Sens Actuators Microsyst Conf. 2017 Jun:2017:135-138. doi: 10.1109/TRANSDUCERS.2017.7994006.

Authors

Vijay Viswam¹, Raziyeh Bounik¹, Amir Shadmani¹, Jelena Dragas¹, Marie Obien^{1

2}, Jan Müller^{1

2}, Yihui Chen^{1

3}, Andreas Hierlemann¹

Affiliations

¹ Bio Engineering Laboratory, ETH Zurich, Basel, Switzerland.
² MaxWell Biosystems AG, Basel, Switzerland.
³ Analog Devices Shanghai Co. Ltd., Shanghai, China.

Abstract

We present a CMOS-based high-density microelectrode array (HD-MEA) system that enables high-density mapping of brain slices in-vitro with multiple readout modalities. The 4.48×2.43 mm² array consists of 59,760 micro-electrodes at 13.5 µm pitch (5487 electrodes/mm²). The overall system features 2048 action-potential, 32 local-field-potential and 32 current recording channels, 32 impedance-measurement and 28 neurotransmitter-detection channels and 16 voltage/current stimulation channels. The system enables real-time and label-free monitoring of position, size, morphology and electrical activity of brain slices.

Keywords: Action potential; Brain slices; CMOS; Electrophysiology; High-density microelectrode arrays; Impedance measurement.

Abstract

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