Photo-controlled aptamers delivery by dual surface gold-magnetic nanoparticles for targeted cancer therapy

Jian Zhao; Keyao Tu; Yanlei Liu; Yulei Qin; Xiwei Wang; Lifeng Qi; Donglu Shi

doi:10.1016/j.msec.2017.04.044

Photo-controlled aptamers delivery by dual surface gold-magnetic nanoparticles for targeted cancer therapy

Mater Sci Eng C Mater Biol Appl. 2017 Nov 1:80:88-92. doi: 10.1016/j.msec.2017.04.044. Epub 2017 Apr 29.

Authors

Jian Zhao¹, Keyao Tu², Yanlei Liu³, Yulei Qin⁴, Xiwei Wang³, Lifeng Qi⁵, Donglu Shi⁶

Affiliations

¹ Key Laboratory of Rubber-Plastics Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastics, Qingdao University of Science & Technology, No. 53 Zhengzhou Road, Qingdao 266042, China; The Materials Science and Engineering Program, Dept. of Mechanical and Materials Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA. Electronic address: zhaojian@gmail.com.
² Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200120, China.
³ Key Laboratory of Rubber-Plastics Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastics, Qingdao University of Science & Technology, No. 53 Zhengzhou Road, Qingdao 266042, China.
⁴ Key Laboratory of Rubber-Plastics Ministry of Education/Shandong Provincial Key Laboratory of Rubber-Plastics, Qingdao University of Science & Technology, No. 53 Zhengzhou Road, Qingdao 266042, China; Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200120, China.
⁵ Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200120, China. Electronic address: lfqi@tongji.edu.cn.
⁶ Shanghai East Hospital, The Institute for Biomedical Engineering & Nano Science, Tongji University School of Medicine, Shanghai 200120, China; The Materials Science and Engineering Program, Dept. of Mechanical and Materials Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA.

PMID: 28866229
DOI: 10.1016/j.msec.2017.04.044

Abstract

Dual surfaced dumbbell-like gold magnetic nanoparticles (Au-Fe₃O₄) were synthesized for targeted aptamers delivery. Their unique biological properties were characterized as a smart photo-controlled drug carrier. DNA aptamers targeting vascular endothelial growth factor (VEGF) were assembled onto the surface of Au-Fe₃O₄ by electrostatic absorption. The binding capacity of the nanoparticles with VEGF aptamers was confirmed by gel electrophoresis. The targeted recognization of ovarian cancer cells by the aptamers-functionalized Au-Fe₃O₄ nanoparticles (Apt-Au-Fe₃O₄ NPs) was observed by confocal microscopy. Apt-Au-Fe₃O₄ was found to bind with SKOV-3 ovarian cancer cells specifically, leading to marked intracellular release of aptamers upon plasmon-resonant light (605nm) radiation, and to enhance the in vitro inhibition against tumor cell proliferation. The results show high potential of Apt-Au-Fe₃O₄as a targeted cancer hyperthermia carrier by remote control with high spatial/temporal resolution.

Keywords: Dumbbell-like gold magnetic nanoparticles aptamers; Ovarian cancer; Photo-control; VEGF.

MeSH terms

Aptamers, Nucleotide
Gold
Humans
Magnetics
Magnetite Nanoparticles*
Neoplasms
Vascular Endothelial Growth Factor A

Substances

Aptamers, Nucleotide
Magnetite Nanoparticles
Vascular Endothelial Growth Factor A
Gold