MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes

Methods Mol Biol. 2017:1659:215-229. doi: 10.1007/978-1-4939-7249-4_19.

Abstract

MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene targeted enrichment sequencing, and computational analysis based on comparative genomics are provided in this chapter.

Keywords: Gene cloning; MutRenSeq; NB-LRR; Resistance gene; Target enrichment sequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular / methods*
  • DNA, Plant / genetics
  • Disease Resistance
  • Genes, Plant*
  • Genomics / methods*
  • Mutation*
  • Plant Diseases / genetics*
  • Plants / genetics*
  • Polymerase Chain Reaction / methods
  • Software

Substances

  • DNA, Plant