Adsorption of cellobiohydrolases I onto lignin fractions from dilute acid pretreated Broussonetia papyrifera

Lan Yao; Haitao Yang; Chang Geun Yoo; Xianzhi Meng; Mi Li; Yunqiao Pu; Arthur J Ragauskas; Robert W Sykes

doi:10.1016/j.biortech.2017.08.024

Adsorption of cellobiohydrolases I onto lignin fractions from dilute acid pretreated Broussonetia papyrifera

Bioresour Technol. 2017 Nov;244(Pt 1):957-962. doi: 10.1016/j.biortech.2017.08.024. Epub 2017 Aug 8.

Authors

Lan Yao¹, Haitao Yang², Chang Geun Yoo³, Xianzhi Meng⁴, Mi Li³, Yunqiao Pu³, Arthur J Ragauskas⁵, Robert W Sykes⁶

Affiliations

¹ School of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, China; Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA.
² School of Pulp & Paper Engineering, Hubei University of Technology, Wuhan 430068, China.
³ Joint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
⁴ Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA.
⁵ Joint Institute for Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA; Department of Chemical and Biomolecular Engineering, The University of Tennessee, Knoxville, TN 37996-2200, USA; Department of Forestry, Wildlife and Fisheries, Center of Renewable Carbon, The University of Tennessee, Institute of Agriculture, Knoxville, TN 37996-2200, USA. Electronic address: aragausk@utk.edu.
⁶ National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, USA.

PMID: 28847086
DOI: 10.1016/j.biortech.2017.08.024

Abstract

Broussonetia papyrifera, known as paper mulberry, is a potential feed stock for bioethanol production because of its cellulose-rich composition. Lignin in dilute acid pretreated Broussonetia papyrifera was fractionated to three different fractions, and their physiochemical properties were determined by FT-IR, GPC and NMR analyses. Different structural characteristics were observed from each lignin fraction. Cellobiohydrolases I (CBH) adsorption to each lignin was understood by the lignin properties. The results showed that aliphatic hydroxyl groups in lignin showed positive correlations with the maximum binding ability of CBH onto lignin samples. Also, the contents of phenolic compounds such as p-hydroxyphenyl benzoate (PB), syringyl (S) and guaiacyl (G) units in the lignin influenced their CBH binding.

Keywords: Adsorption; Broussonetia papyrifera; Cellobiohydrolases I; Dilute acid pretreatment; Lignin fraction.

MeSH terms

Adsorption
Broussonetia*
Cellulase
Cellulose 1,4-beta-Cellobiosidase*
Hydrolysis
Lignin
Spectroscopy, Fourier Transform Infrared

Substances

Lignin
Cellulase
Cellulose 1,4-beta-Cellobiosidase